Optical Imaging

Optical Imaging techniques, such as Bioluminescence imaging (BLI) and Fluorescence imaging (FLI) are non-invasive modality which are important tools in biomedical research. These techniques allow longitudinal monitoring of subjects, and enable visualisation of physiological processes at the cellular and molecular level in-vivo with high specificity.

Bioluminescence imaging (BLI) is a non-invasive imaging modality widely used in the field of pre-clinical oncology research. Imaging of small animal tumour models using BLI involves the generation of light by luciferase-expressing cells in the animal following administration of the substrate Luciferin. This light may be imaged using an external detector, such as the IVIS Spectrum (Perkin Elmer). The technique allows a variety of tumour-associated properties to be visualized dynamically in living models. The increasing use of BLI as a small-animal imaging modality has led to advances in the development of xenogeneic, orthotopic, and genetically engineered animal models expressing luciferase genes.

Fluorescence imaging (FI) is a type of non-invasive imaging that can help visualise biological processes taking place in a living organism. FI utilises fluorescence that results from molecules (known as fluorophores) emitting light of a certain wavelength (300-80nm) after absorbance of electromagnetic radiation. This light can be detected and quantified using an external detector, such as the IVIS Spectrum (Perkin Elmer).